USE: Total Vitamin D or 25-Hydroxyvitamin D level is the best indicator of overall Vitamin D status because it reflects total Vitamin D from dietary intake, sunlight exposure and conversion of Vitamin D from adipose stores in the liver. Vitamin D deficiency may lead to muscle weakness, bone pain and fragility fractures. Insufficiency may be associated with both skeletal and nonskeletal health outcomes eg. atherosclerosis, diabetes Mellitus (Type 1 and 2), multiple sclerosis and other autoimmune disorders, neoplasms and cardiovascular diseases. Use of Test: Assessment of deficiency or insufficiency of Vitamin D